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KMID : 0380620200520010026
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Korean Journal of Food Science and Technology
2020 Volume.52 No. 1 p.26 ~ p.30
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Isolation and HPLC-DAD validation of xanthoangelol in Lespedeza bicolor extract
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Woo Hyun-Sim
Kim Yeong-Su
Oh Yu-Jin
Cho Hae-Jin
Song Se-Kyu
Kim Dae-Wook
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Abstract
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This study was undertaken to determine the characteristics of xanthoangelol, the major chalcone constituent derived from the extracts of different parts of Lespedeza bicolor. Xanthoangelol was isolated from the root extract using column chromatography and used as a standard for quantitative analysis. The structure of the isolated compound was established based on spectroscopic evidence. The HPLC-DAD method was validated for specificity, linearity, precision, accuracy, limit of detection, and limit of quantitation. The calibration curve of xanthoangelol had significant linearity (R2>0.9999). Limit of detection and limit of quantitation 0.018 and 0.059 μg/mL, respectively. The relative standard deviation values of precision test, and intra- and inter-day tests were less than 0.22 and 0.40%, respectively. In the recovery test, the accuracy ranged from 98.98-102.78% with RSD values less than 0.13%. The method validation parameters indicate the applicability of the HPLC method for quality control of food or drug formulations containing L. bicolor.
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KEYWORD
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Lespdeza bicolor, chalcone, xanthoangelol, HPLC-DAD, validation
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